Impact of phenylpropanoid compounds on heat stress tolerance in carrot cell cultures

The phenylpropanoid and flavonoid families include thousands of specialized metabolites that influence a wide range of processes in plants, including seed dispersal, auxin transport, photoprotection, mechanical support and protection against insect herbivory. Such metabolites play a key role in the protection of plants against abiotic stress, in many cases through their well-known ability to inhibit the formation of reactive oxygen species (ROS). However, the precise role of specific phenylpropanoid and flavonoid molecules is unclear. We therefore investigated the role of specific anthocyanins (ACs) and other phenylpropanoids that accumulate in carrot cells cultivated in vitro, focusing on their supposed ability to protect cells from heat stress. First we characterized the effects of heat stress to identify quantifiable morphological traits as markers of heat stress susceptibility. We then fed the cultures with precursors to induce the targeted accumulation of specific compounds, and compared the impact of heat stress in these cultures and unfed controls. Data modeling based on Projection to Latent Structures (PLS) regression revealed that metabolites containing coumaric or caffeic acid, including ACs, correlate with less heat damage. Further experiments suggested that one of the cellular targets damaged by heat stress and protected by these metabolites is the actin microfilament cytoskeleton

Año Internacional de la Astronomia: "Galileo, entre la ciencia y la fe" : El aprendizaje por proyectos

Uno de los fines de las Ciencias Naturales es ofrecer al estudiante de escuela secundaria, las herramientas metodológicas específicas del área, a fin de que los saberes de la materia puedan ser aplicados, en la construcción de otros conocimientos, igual o más complejos y también en las acciones de la vida cotidiana. El año 2009, declarado por las Naciones Unidad como el Año Internacional de la Astronomía, en honor a las primeras observaciones de Galileo, es excelente oportunidad para que, a través del trabajo mediante proyectos, se revalorice su tarea, sus aportes a la Ciencia, y se realice una lectura crítica de su relación con la Iglesia y las instituciones de la época. Partiendo de los contenidos del curriculum para los primeros, segundos y terceros años de la Escuela Secundaria, este proyecto intenta, un rastreo bibliográfico, como así también una profundización experimental en lo que atañe a la observación y reconocimiento de astros, trabajos realizados por Galileo, experimentación con lentes y construcción de telescopios sencillos. También se extiende la tarea interdisciplinar, al área de informática, con el armado y exposición de presentaciones de power- point por parte de los alumnos de los distintos años. Finalmente, teniendo presente la importancia ineludible del trabajo de la escuela en unión con las familias y la comunidad, se organiza un concurso de ensayos, para adultos, sobre la vida de este astrónomo y físico, con la participación de padres y docentes, abierto a otras escuelas e instituciones.Trabajos del área Ciencias ExactasDepartamento de Ciencias Exactas y Naturale

Leicester Water District Analysis

Delivering high quality drinking water to the residents of a town or city necessitates a robust water management system to ensure the health and safety of its residents. Our report investigates the challenges faced by the water supply districts in the Town of Leicester, Massachusetts. By working together with the Massachusetts Department of Environmental Protection and the Town of Leicester, our goal was to analyze the feasibility of incorporating a collaborative water management system in the Town of Leicester to help alleviate issues of water quality and quantity. In our report, we provide recommendations for the Town of Leicester, its water districts and the Moose Hill Commission on how to collaborate to help Leicester with its current and future water needs

The Chemical Origin of SEY at Technical Surfaces

The secondary emission yield (SEY) properties of colaminated Cu samples for LHC beam screens are correlated to the surface chemical composition determined by X-ray photoelectron spectroscopy. The surface of the "as received" samples is characterized by the presence of significant quantities of contaminating adsorbates and by the maximum of the SEY curve (dmax) being as high as 2.2. After extended electron scrubbing at kinetic energy of 10 and 500 eV, the dmax value drops to the ultimate values of 1.35 and 1.1, respectively. In both cases the surface oxidized phases are significantly reduced, whereas only in the sample scrubbed at 500 eV the formation of a graphitic-like C layer is observed. We find that the electron scrubbing of technical Cu surfaces can be described as occurring in two steps, where the first step consists in the electron induced desorption of weakly bound contaminants that occurs indifferently at 10 and at 500 eV and corresponds to a partial decrease of dmax, and the second step, activated by more energetic electrons and becoming evident at high doses, which increases the number of graphitic-like C-C bonds via the dissociation of adsorbates already contaminating the "as received" surface or accumulating on this surface during irradiation. Our results demonstrate how the kinetic energy of impinging electrons is a crucial parameter when conditioning technical surfaces of Cu and other metals by means of electron induced chemical processing.Comment: 6 pages, contribution to the Joint INFN-CERN-EuCARD-AccNet Workshop on Electron-Cloud Effects: ECLOUD'12; 5-9 Jun 2012, La Biodola, Isola d'Elba, Italy; CERN Yellow Report CERN-2013-002, pp.99-10

Undifferentiated In Vitro Cultured Actinidia deliciosa as Cell Factory for the Production of Quercetin Glycosides

Land plants produce a vast arsenal of specialized metabolites and many of them display interesting bioactivities in humans. Recently, flavonol quercetin gained great attention in the light of the COVID-19 pandemic because, in addition to the anti-inflammatory, antiviral and anti-cancer activity already described, it emerged as possible inhibitor of 3CLpro, the major protease of SARS-CoV-2 virus. Plant cell and tissue culture (PCTC) is an attractive platform for the biotechnological production of plant metabolites. This technology allows a large amount of water and agricultural land to be saved and, being free of contaminants in the process, it is suitable for scaling up the production in bioreactors. In a project aimed to generate and screen in vitro plant cells for the production of valuable specialized metabolites for commercial production, we generated various cell lines from Actinidia deliciosa (kiwi fruit tree) and Actinidia chinensis (gold kiwi fruit tree), that were able to produce relevant amounts of quercetin derivatives, mainly quercetin glycosides. Three cell lines from A. deliciosa were characterized by targeted and untargeted metabolomics. In standard growing conditions, they produce and accumulate up to 13.26 mg/100 g fresh weight (419.76 mg/100 g dry weight) of quercetin derivatives. To address future industrial applications, these cell lines should be entered into an acceleration program to further increase the amount of these metabolites by optimizing the culture conditions and elicitation

Advances in combined enzymatic extraction of ferulic acid from wheat bran

Wheat bran could be utilised as feedstock for innovative and sustainable biorefinery processes. Here, an enzymatic hydrolysis process for ferulic acid (FA) extraction was optimised step by step for total wheat bran (Tritello) and then also applied to the outer bran layer (Bran 1). Proteins, reducing sugars, total phenols and FA were quantified. The highest FA yields (0.82-1.05\u202fg/kg bran) were obtained either by rehydrating the bran by autoclaving (Tritello) or by steam explosion (Bran 1) using a bran/water ratio of 1:20, followed by enzymatic pre-treatment with Alcalase and Termamyl, to remove protein and sugars, and a final enzymatic hydrolysis with Pentopan and feruloyl esterase to solubilise phenol. FA was recovered from the final digestate via solid phase extraction. A 40-fold scale-up was also performed and the release of compounds along all the process steps and at increasing incubation times was monitored. Results showed that FA was initially present at a minimum level while it was specifically released during the enzymatic treatment. In the final optimized process, the FA extraction yield was higher than that obtained with NaOH control hydrolysis while, in comparison with other FA enzymatic extraction methods, fewer process steps were required and no buffers, strong acid/alkali nor toxic compounds were used. Furthermore, the proposed process may be easily scaled-up, confirming the feasibility of wheat bran valorisation by biorefinery processes to obtain valuable compounds having several areas of potential industrial exploitation

The role of atomic collisions in kinetic electron emission from Al surfaces by slow ions

Abstract We measured energy distributions of electrons emitted in the interaction of slow Kr + and Na + with Al surfaces. The data allow to correlate emission intensities with spectral signatures of electron excitation processes. Our results indicate that electron promotion processes leading to the excitation of Al target atoms plays the dominating role in kinetic electron emission from Al surfaces by slow ions. In the case of Kr + ions, electron promotion occurs in close atomic collisions between recoiling target atoms. For Na + projectiles, a significant contribution to Al excitation comes also from a vacancy transfer process in asymmetric collisions involving ions that have survived neutralization in the interaction with the surface

Kinetic electron emission from Al surfaces by slow ions

We measured energy distributions of electrons emitted in the interaction of Na + with Al surfaces at incident ion energies in the range 150– 4000 eV. The data allow to correlate emission intensities with spectral signatures of electron excitation processes. We find a remarkable contribution to electron emission from asymmetric collisions between incoming ions that have survived neutralization at the surface and target atoms, leading to Al-2p excitation via a vacancy transfer process. We observe that the total electron emission yields sharp increases by more than an order of magnitude at impact energies above the threshold for this process

Status of COLDDIAG: A Cold Vacuum Chamber for Diagnostics

One of the still open issues for the development of superconducting insertion devices is the understanding of the beam heat load. With the aim of measuring the beam heat load to a cold bore and the hope to gain a deeper understanding in the beam heat load mechanisms, a cold vacuum chamber for diagnostics is under construction. The following diagnostics will be implemented: i) retarding field analyzers to measure the electron energy and flux, ii) temperature sensors to measure the total heat load, iii) pressure gauges, iv) and mass spectrometers to measure the gas content. The inner vacuum chamber will be removable in order to test different geometries and materials. This will allow the installation of the cryostat in different synchrotron light sources. COLDDIAG will be built to fit in a short straight section at ANKA. A first installation at the synchrotron light source Diamond is foreseen in June 2011. Here we describe the technical design report of this device and the planned measurements with beam.Comment: Presented at First International Particle Accelerator Conference, IPAC'10, Kyoto, Japan, from 23 to 28 May 201

PIP3-dependent macropinocytosis is incompatible with chemotaxis

In eukaryotic chemotaxis, the mechanisms connecting external signals to the motile apparatus remain unclear. The role of the lipid phosphatidylinositol 3,4,5-trisphosphate (PIP3) has been particularly controversial. PIP3 has many cellular roles, notably in growth control and macropinocytosis as well as cell motility. Here we show that PIP3 is not only unnecessary for Dictyostelium discoideum to migrate toward folate, but actively inhibits chemotaxis. We find that macropinosomes, but not pseudopods, in growing cells are dependent on PIP3. PIP3 patches in these cells show no directional bias, and overall only PIP3-free pseudopods orient up-gradient. The pseudopod driver suppressor of cAR mutations (SCAR)/WASP and verprolin homologue (WAVE) is not recruited to the center of PIP3 patches, just the edges, where it causes macropinosome formation. Wild-type cells, unlike the widely used axenic mutants, show little macropinocytosis and few large PIP3 patches, but migrate more efficiently toward folate. Tellingly, folate chemotaxis in axenic cells is rescued by knocking out phosphatidylinositide 3-kinases (PI 3-kinases). Thus PIP3 promotes macropinocytosis and interferes with pseudopod orientation during chemotaxis of growing cells